Chan Lab:

Autophagy nutrient sensing

Nwadike C., Williamson L.E., Gallagher L.E. , Guan J.-L., Chan E.Y.W. (2018) AMPK inhibits ULK1-dependent autophagosome formation and lysosomal acidification via distinct mechanisms.  Mol Cell Biol.  00023-18. doi: 10.1128/MCB.00023-18    

Gallagher, L. E., Radhi, O. A., Abdullah, M. O., McCluskey, A. G., Boyd, M. and Chan, E. Y. W. (2017). Lysosomotropism depends on glucose: a chloroquine resistance mechanism. Cell Death Dis, 8(8), e3014. doi:10.1038/cddis.2017.416.   

Mitchell, R., Hopcroft, L. E. M., Baquero, P., Allan, E. K., Hewit, K., James, D., Hamilton, G., Mukhopadhyay, A., O'Prey, J., Hair, A., Melo, J. V., Chan, E., Ryan, K. M., Maguer-Satta, V., Druker, B. J., Clark, R. E., Mitra, S., Herzyk, P., Nicolini, F. E., Salomoni, P. and Helgason, G. V. (2017). Targeting BCR-ABL-Independent TKI Resistance in Chronic Myeloid Leukemia by mTOR and Autophagy Inhibition. J Natl Cancer Inst. doi:10.1093/jnci/djx236

Karvela, M., Baquero, P., Kuntz, E. M., Mukhopadhyay, A., Mitchell, R., Allan, E. K., Chan, E., Kranc, K. R., Calabretta, B., Salomoni, P., Gottlieb, E., Holyoake, T. L. and Helgason, G. V. (2016). ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells. Autophagy, 12(6), 936-948. doi:10.1080/15548627.2016.1162359.   

Gallagher, L., Williamson, L. and Chan, E. (2016). Advances in Autophagy Regulatory Mechanisms. Cells, 5(2), 24. doi:10.3390/cells5020024.

Klionsky, D. J., et al. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222. doi:10.1080/15548627.2015.1100356.   

Watson, D. G., Tonelli, F., Alossaimi, M., Williamson, L., Chan, E., Gorshkova, I., Berdyshev, E., Bittman, R., Pyne, N. J. and Pyne, S. (2013). The roles of sphingosine kinases 1 and 2 in regulating the Warburg effect in prostate cancer cells. Cell Signal, 25(4), 1011-1017. doi:10.1016/j.cellsig.2013.01.002.

Tonelli, F., Alossaimi, M., Williamson, L., Tate, R. J., Watson, D. G., Chan, E., Bittman, R., Pyne, N. J. and Pyne, S. (2013). The sphingosine kinase inhibitor 2-(p-hyroxyanilino)-4-(p-chlorophenyl)thiazole reduces androgen receptor expression via an oxidative stress-dependent mechanism. Br J Pharmacol, 168(6), 1497-1505. doi:10.1111/bph.12035.

Mleczak, A., Millar, S., Tooze, S. A., Olson, M. F. and Chan, E. Y. (2013). Regulation of autophagosome formation by Rho kinase. Cell Signal, 25(1), 1-11. doi:10.1016/j.cellsig.2012.09.010.  

McAlpine, F., Williamson, L. E., Tooze, S. A. and Chan, E. Y. (2013). Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2. Autophagy, 9(3), 361-373. doi:10.4161/auto.23066.  

Gallagher, L. E. and Chan, E. Y. (2013). Early signalling events of autophagy. Essays Biochem, 55, 1-15. doi:10.1042/bse0550001.   

Klionsky, D. J., et al. (2012). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 8(4), 445-544.   

PMID: 22966490

Chan, E. Y. (2012). Regulation and function of uncoordinated-51 like kinase proteins. Antioxid Redox Signal, 17(5), 775-785. doi:10.1089/ars.2011.4396.   

Razi, M., Chan, E. Y. and Tooze, S. A. (2009). Early endosomes and endosomal coatomer are required for autophagy. J Cell Biol, 185(2), 305-321. doi:10.1083/jcb.200810098.

Chan, E. Y. and Tooze, S. A. (2009). Evolution of Atg1 function and regulation. Autophagy, 5(6), 758-765.

Chan, E. Y., Longatti, A., McKnight, N. C. and Tooze, S. A. (2009). Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism. Mol Cell Biol, 29(1), 157-171. doi:10.1128/mcb.01082-08.   

Chan, E. Y. (2009). mTORC1 phosphorylates the ULK1-mAtg13-FIP200 autophagy regulatory complex. Sci Signal, 2(84), pe51. doi:10.1126/scisignal.284pe51.

Chan, E. Y., Kir, S. and Tooze, S. A. (2007). siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem, 282(35), 25464-25474. doi:10.1074/jbc.M703663200.

Young, A. R., Chan, E. Y., Hu, X. W., Kochl, R., Crawshaw, S. G., High, S., Hailey, D. W., Lippincott-Schwartz, J. and Tooze, S. A. (2006). Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes. J Cell Sci, 119(Pt 18), 3888-3900. doi:10.1242/jcs.03172.

Kochl, R., Hu, X. W., Chan, E. Y. and Tooze, S. A. (2006). Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes. Traffic, 7(2), 129-145. doi:10.1111/j.1600-0854.2005.00368.x.

Warby, S. C., Chan, E. Y., Metzler, M., Gan, L., Singaraja, R. R., Crocker, S. F., Robertson, H. A. and Hayden, M. R. (2005). Huntingtin phosphorylation on serine 421 is significantly reduced in the striatum and by polyglutamine expansion in vivo. Hum Mol Genet, 14(11), 1569-1577. doi:10.1093/hmg/ddi165.   

Tang, T. S., Tu, H., Orban, P. C., Chan, E. Y., Hayden, M. R. and Bezprozvanny, I. (2004). HAP1 facilitates effects of mutant huntingtin on inositol 1,4,5-trisphosphate-induced Ca release in primary culture of striatal medium spiny neurons. Eur J Neurosci, 20(7), 1779-1787. doi:10.1111/j.1460-9568.2004.03633.x.   

Tang, T. S., Tu, H., Chan, E. Y., Maximov, A., Wang, Z., Wellington, C. L., Hayden, M. R. and Bezprozvanny, I. (2003). Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1,4,5) triphosphate receptor type 1. Neuron, 39(2), 227-239.   

Luthi-Carter, R., Hanson, S. A., Strand, A. D., Bergstrom, D. A., Chun, W., Peters, N. L., Woods, A. M., Chan, E. Y., Kooperberg, C., Krainc, D., Young, A. B., Tapscott, S. J. and Olson, J. M. (2002). Dysregulation of gene expression in the R6/2 model of polyglutamine disease: parallel changes in muscle and brain. Hum Mol Genet, 11(17), 1911-1926.   

Chan, E. Y., Stang, S. L., Bottorff, D. A. and Stone, J. C. (2002). Mutations in conserved regions 1, 2, and 3 of Raf-1 that activate transforming activity. Mol Carcinog, 33(4), 189-197.   

Chan, E. Y., Nasir, J., Gutekunst, C. A., Coleman, S., Maclean, A., Maas, A., Metzler, M., Gertsenstein, M., Ross, C. A., Nagy, A. and Hayden, M. R. (2002). Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet, 11(8), 945-959.   

Chan, E. Y., Luthi-Carter, R., Strand, A., Solano, S. M., Hanson, S. A., DeJohn, M. M., Kooperberg, C., Chase, K. O., DiFiglia, M., Young, A. B., Leavitt, B. R., Cha, J. H., Aronin, N., Hayden, M. R. and Olson, J. M. (2002). Increased huntingtin protein length reduces the number of polyglutamine-induced gene expression changes in mouse models of Huntington's disease. Hum Mol Genet, 11(17), 1939-1951.   

Chan, E. Y. (2002). HIP1 as a marker of aggressive prostate cancer. Clin Genet, 62(5), 372-375.   

Hanna, A. N., Chan, E. Y., Xu, J., Stone, J. C. and Brindley, D. N. (1999). A novel pathway for tumor necrosis factor-alpha and ceramide signaling involving sequential activation of tyrosine kinase, p21(ras), and phosphatidylinositol 3-kinase. J Biol Chem, 274(18), 12722-12729.   

Chan, E. Y., Stang, S. L., Bottorff, D. A. and Stone, J. C. (1999). Hypothermic stress leads to activation of Ras-Erk signaling. J Clin Invest, 103(9), 1337-1344. doi:10.1172/jci5474.   

Ebinu, J. O., Bottorff, D. A., Chan, E. Y., Stang, S. L., Dunn, R. J. and Stone, J. C. (1998). RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. Science, 280(5366), 1082-1086.   

Rusinol, A. E., Chan, E. Y. and Vance, J. E. (1993). Movement of apolipoprotein B into the lumen of microsomes from hepatocytes is disrupted in membranes enriched in phosphatidylmonomethylethanolamine. J Biol Chem, 268(33), 25168-25175.