| Title | Growth factor mediated trafficking of the tumour suppressor Sprouty-2 |
|---|---|
| Supervisors | Luke Chamberlain Gwyn Gould |
| Research Area | Cellular basis of disease, membrane trafficking |
| Description | Sprouty-2 is an important regulator of growth factor (FGF, EGF) signalling pathways and disruption of this protein is linked to cancer. Sprouty-2 has been shown to translocate from an intracellular pool to the plasma membrane upon growth factor stimulation. The minimal translocation domain of Sprouty-2 includes a highly palmitoylated cysteine-rich region, which regulates membrane interactions of Sprouty-2. The movement of Sprouty-2 to the plasma membrane in response to growth factors is thought to be important for regulation of growth factor signalling, however there is very little known about the mechanisms controlling Sprouty-2 translocation or the role of palmitoylation in this process. The aim of this project is therefore to define the trafficking pathway that mediates movement of Sprouty-2 to the plasma membrane in response to growth factors and to determine the importance of palmitoylation for this process. |
| Techniques Used | The project will use a range of techniques including confocal microscopy, click chemistry and CRISPR. |
| References | Greaves J, Munro KR, Davidson SC, Riviere M, Wojno J, Smith TK, Tomkinson NC, Chamberlain LH. Proc Natl Acad Sci U S A. 2017 114(8):E1365-E1374. Chamberlain LH, Shipston MJ. Physiol Rev. 2015 95(2):341-76 |
| Conditions | Applicants should possess or be about to obtain a 1st class or 2:1 Honours degree or equivalent in a relevant discipline in addition to receipt of satisfactory references and an IELTS score of 6.5 where appropriate. |
| Bench Fee | Running costs of £10000 p.a. will be associated with this project in addition to University tuition fees. |
| Suitable For | students with background in biochemistry or cell biology |
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