I obtained my BSc (Hons) in Pharmacology from the University of Glasgow (1992) and my PhD in Neuroscience from the University of Bristol (1996) before undertaking postdoctoral research at the University of Chicago and Imperial College London. I took up my position here at Strathclyde in 2002.
My research interest lies primarily in the field of neuroscience and focuses on the modulation of neuronal and excitability and synaptic transmission in both physiological and pathophysiological conditions. This currently takes several directions: the role of proteinase-activated receptors in central neurones, the G-protein coupled receptor modulation of two-pore K+ channels (K2Ps), cannabinoid modulation of neuronal activity and immune cell involvement in neurological disorders. A variety of preparations are utilised in our research including mammalian expression systems, cultured neurones, organotypic slice cultures and acute brain slices. Techniques used include cell death assays, electrophysiology, immunocytochemistry, overexpression of fluorescently tagged proteins of interest and molecular biological techniques including site-directed mutagenesis and real time RT-PCR.
I am involved in teaching both the MPharm and BMS cohorts in numerous classes ranging from 2nd to 4th year.
I am currently a committee member and active member of the British Neuroscience Association, the local representative and member of the Physiological Society and the Strathclyde representative for the Scottish Neuroscience Group. I am also a full member of the Society for Neuroscience and a Fellow of the Higher Education Academy.
"Determining the physiological and pathophysiological roles of neuronal PAR-2" Wellcome Trust
Publications
Fumagalli, S, Coles, JAC, Ejlerskov, P., Ortolano, F., Bushell, T. J., Brewer, J.M., De Simoni, M.G., Dever, G., Garside, P., Maffia, P., Carswell, H.V.
(2011) In vivo Real Time Imaging of T Lymphocyte Dynamics in Mouse Brain by Multiphoton Microscopy After Stroke.. Stroke42, 1429-1436
Gan J, Greenwood S, Cobb S and Bushell T (2011) Indirect modulation of neuronal excitability and synaptic transmission in the hippocampus by activation of proteinase-activated receptor-2. British Journal of Pharmacology163, 984-994 [DOI: 10.1111/j.1476-5381.2011.01293.x] Published (review)
Ledgerwood CJ, Greenwood SM, Brett RR, Pratt JA, Bushell TJ (2011) Cannabidiol inhibits synaptic transmission in rat hippocampal cultures and slices via several receptor pathways.. British Journal of Pharmacology162, 286-294
Ortolano, F., Maffia, P., Dever, G., Rodolico, G., Millington, O.R., De Simoni, M.G., Brewer, J.M., Bushell, T.J., Garside, P. and Carswell H.V. (2010) Advances in imaging of new targets for pharmacological intervention in stroke: real time tracking of T cells in the ischaemic brain. British Journal of Pharmacology159, 808-811
Greenwood, S.M. and Bushell, T.J. (2010) Astrocytic activation and an inhibition of MAP kinases are required for proteinase-activated receptor-2-mediated protection from neurotoxicity.. Journal of Neurochemistry113, 1471-1480
Shakya-Shrestha, S., Parmar, M., Kennedy, C. and Bushell, T.J. (2010) Two-pore potassium ion channels are inhibited by both Gq/11- and Gi-coupled P2Y receptors.. Molecular and Cellular Neuroscience43, 363-369 [DOI: 10.1016/j.mcn.2010.01.003]
Fumagalli, S, Coles, JAC, Ejlerskov, P., Ortolano, F., Bushell, T. J., Brewer, J.M., De Simoni, M.G., Dever, G., Garside, P., Maffia, P., Carswell, H.V.
(2011) In vivo Real Time Imaging of T Lymphocyte Dynamics in Mouse Brain by Multiphoton Microscopy After Stroke.. Stroke42, 1429-1436
Gan J, Greenwood S, Cobb S and Bushell T (2011) Indirect modulation of neuronal excitability and synaptic transmission in the hippocampus by activation of proteinase-activated receptor-2. British Journal of Pharmacology163, 984-994 [DOI: 10.1111/j.1476-5381.2011.01293.x] Published (review)
Ledgerwood CJ, Greenwood SM, Brett RR, Pratt JA, Bushell TJ (2011) Cannabidiol inhibits synaptic transmission in rat hippocampal cultures and slices via several receptor pathways.. British Journal of Pharmacology162, 286-294
Ortolano, F., Maffia, P., Dever, G., Rodolico, G., Millington, O.R., De Simoni, M.G., Brewer, J.M., Bushell, T.J., Garside, P. and Carswell H.V. (2010) Advances in imaging of new targets for pharmacological intervention in stroke: real time tracking of T cells in the ischaemic brain. British Journal of Pharmacology159, 808-811
Greenwood, S.M. and Bushell, T.J. (2010) Astrocytic activation and an inhibition of MAP kinases are required for proteinase-activated receptor-2-mediated protection from neurotoxicity.. Journal of Neurochemistry113, 1471-1480
Shakya-Shrestha, S., Parmar, M., Kennedy, C. and Bushell, T.J. (2010) Two-pore potassium ion channels are inhibited by both Gq/11- and Gi-coupled P2Y receptors.. Molecular and Cellular Neuroscience43, 363-369 [DOI: 10.1016/j.mcn.2010.01.003]
Group
Postdoctoral researchers
Dr Sam Greenwood: 'Determining the physiological and pathophysiological roles of neuronal PAR-2' (started 2008)
Postgraduate students
Roua Abulkassim: 'Determining the role of proteinase activated receptor-2 in mouse behaviour' (started 2009)
Conor Doherty: 'Does activation of toll-like receptor 3 (TLR 3) modulate hippocampal function under physiological and pathophysiological conditions?' (started 2010)
David Gavin Glass: 'Quantifying the interaction forces present in the immune system using optical tweezers' (started 2010)
Rajeev Shrestha: 'Do T-cells modulate neuronal activity and plasticity in the CNS durign chronic inflammatory disease?' (started 2008)
