Graduated B.Sc. (Hons.) in Physiology (Glasgow University, 1979), M.Sc. (Medical Science) (Glasgow University, 1984), and Ph.D. “The computer analysis of electrophysiological signals” (Strathclyde University, 1973). Member of the Physiological Society and the Higher Education Academy.
Departmental Responsibilities Manager of SIPBS I.T. support group. Class Coordinator of HP421 (Advanced Statistics) and 59501 (Experimental & Computational Methods in Neuroscience). Lecturer on statistical and data analysis. Developer of computer-based simulations and data acquisition systems for undergraduate pharmacology classes.
Research Interests The development of data acquisition systems and software for acquiring and analyzing electrophysiological signals and images. Author of the ‘Strathclyde Electrophysiology Software’ package - a suite of open source, computer programs for electrophysiological signal and image acquisition. The software, provided free of charge to academic and non-commercial users, is used in many laboratories throughout the world and is currently cited in over 500 publications. Current work focuses upon the development of packages which combine the acquisition of fluorescence images (using both camera and confocal microscopy) with electrophysiological signals.
The software has been developed in collaboration with a number of research groups and has lead to a range of joint publications: Prof. G.L. Smith & Dr. A. Workman, Glasgow University (simulation of cardiac cell potentials and Ca dynamics); Dr. T Carter, NIMR, Mill Hill (imaging of secretion from endothelial cells); Dr. D. Wokosin, University of Chicago (imaging of dendritic synaptic activity using 2 photon laser scanning microscopy, combined with patch clamp recording and stimulation of the neuronal cell body); Dr. B. Zinselmeyer, NIH (tracking of T cells).
Publications
Knipe L, Meli A, Hewlett L, Bierings R, Dempster J, Skehel P, Hannah MJ, Carter T (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells. Blood116, 2183-2191 [DOI: 10.1182/blood-2010-03-276170]
Gelman AE, Li W, Richardson SB, Zinselmeyer BH, Lai J, Okazaki M, Kornfeld CG, Kreisel FH, Sugimoto S, Tietjens JR, Dempster J, Patterson GA, Krupnick AS, Miller MJ, Kreisel D (2009) Cutting edge: Acute lung allograft rejection is independent of secondary lymphoid organs. Journal of Immunology182, 3969-3973 [DOI: 10.4049/jimmunol.0803514]
Babich V, Knipe L, Hewlett L, Meli A, Dempster J, Hannah MJ, Carter T (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body. Journal of Biological Chemistry284, 12459-12468 [DOI: 10.1074/jbc.M809235200]
MacQuaide N, Dempster J, Smith GL (2009) Assessment of sarcoplasmic reticulum Ca2+ depletion during spontaneous Ca2+ waves in isolated permeabilized rabbit ventricular cardiomyocytes. Biophysical Journal96, 2744-2754 [DOI: 10.1016/j.bpj.2008.12.3944]
Marshall G., Russell J., Tellez J., Jhund P., Currie S., Dempster J., Boyett M., Kane K., Rankin A & Workman A. (2011) Remodelling of human atrial K+ currents but not ion channel expression by chronic blockade. Pflügers Archiv: European Journal of Physiology , In Press
Zinselmeyer BH, Dempster J, Wokosin DL, Cannon JJ, Pless R, Parker I, Miller MJ (2009) Two-photon microscopy and multidimensional analysis of cell dynamics. Methods in Enzymology461, 349-378 [DOI: 10.1016/S0076-6879(09)05416-0]
Knipe L, Meli A, Hewlett L, Bierings R, Dempster J, Skehel P, Hannah MJ, Carter T (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells. Blood116, 2183-2191 [DOI: 10.1182/blood-2010-03-276170]
Gelman AE, Li W, Richardson SB, Zinselmeyer BH, Lai J, Okazaki M, Kornfeld CG, Kreisel FH, Sugimoto S, Tietjens JR, Dempster J, Patterson GA, Krupnick AS, Miller MJ, Kreisel D (2009) Cutting edge: Acute lung allograft rejection is independent of secondary lymphoid organs. Journal of Immunology182, 3969-3973 [DOI: 10.4049/jimmunol.0803514]
Babich V, Knipe L, Hewlett L, Meli A, Dempster J, Hannah MJ, Carter T (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body. Journal of Biological Chemistry284, 12459-12468 [DOI: 10.1074/jbc.M809235200]
MacQuaide N, Dempster J, Smith GL (2009) Assessment of sarcoplasmic reticulum Ca2+ depletion during spontaneous Ca2+ waves in isolated permeabilized rabbit ventricular cardiomyocytes. Biophysical Journal96, 2744-2754 [DOI: 10.1016/j.bpj.2008.12.3944]
Marshall G., Russell J., Tellez J., Jhund P., Currie S., Dempster J., Boyett M., Kane K., Rankin A & Workman A. (2011) Remodelling of human atrial K+ currents but not ion channel expression by chronic blockade. Pflügers Archiv: European Journal of Physiology , In Press
Zinselmeyer BH, Dempster J, Wokosin DL, Cannon JJ, Pless R, Parker I, Miller MJ (2009) Two-photon microscopy and multidimensional analysis of cell dynamics. Methods in Enzymology461, 349-378 [DOI: 10.1016/S0076-6879(09)05416-0]
Kueha S.L.L., Dempster J., Head S.I., and Morley J.W. (2011) Reduced postsynaptic GABAA receptor number and enhanced gaboxadol induced change in holding currents in Purkinje cells of the dystrophin-deficient mdx mouse. Neurobiology of Disease43, 558-564 [DOI: 10.1016/j.nbd.2011.05.002]
