WinFluor V4.1.5 03/02/20 

Bug Fixes:  

Cameras: Andor Zyla and Neo cameras. Delay added after camera stop to determine whether rapid stop/start of camera leads to intermittent hang ups.

NI Interfaces: Extra space allocated for A/D channel lists to accommodate devices with large no.s of channels.

WinFluor V4.1.4 29/01/20 

Bug Fixes: 

Cameras: Andor Zyla and Neo cameras. DLL libraries now loaded directly from C:\Program Files\Andor SDK3. Modifications to acquisition buffer monitor thread made to avoid system locking up at random intervals when camera acquisition is stopped and restarted.

Amplifiers: A-M Systems 2400. Gain telegraph code updated to handle telegraph voltage series 0,0.3,0.8V and 0,0.5,1.0V ... produced by different versions of the amplifier.

WinFluor V4.1.3 05/12/19 

Bug Fixes:

Cameras: 64 bit version of National Instruments IMAQdx library now work correctly, allowing frame capture interval to be set correctly.

Laboratory Interfaces: Bug which prevented number of analogue input and output channels from being correctly detected with 64 bit version of NIDAQmx 19.5 drivers now fixed.

WinFluor V4.0.6 8/10/18

Bug Fix

Record Images & Signals: Size of light source digital output control buffer now initialised correctly.

WinFluor V4.0.5 29/8/18

Bug Fix

Sutter DG-4 Light Source: Digital bit pattern for filters 1-4 now set correctly. Fourth control line added to Setup panel. Support for 50% and 33% attenuated filter settings added.

WinFluor V4.0.4 28/8/18

Updates

Cameras: Support for PCO cameras added. Tested using PCO Edge 5.5 USB3.

Line Profile Analysis: Edge tracking plot can now be cancelled by user when plotting is in progress. 

WinFluor V4.0.3 15/7/18

Bug Fix

ROI Export: Export of more than 16 ROI time course data sets to ABF file now works correctly. ROIs now stored as records in ABF, WCP and CFS files instead of channels, removing 16 ROI limit. Export to EDR files removed and replace with WCP files. ROIs now stored as concatenated sequence in IBW files instead ot separate files.

WinFluor V3.9.9 26/3/18

Bug Fix

Record Images & Signals: PMT Ratio Calculation: Channel selected as Concentration Output Channel no longer sets the input channel of the same number to zero when Concentration option is disabled.

no longer set to

WinFluor V3.9.7 15/1/18

Bug Fixes 

Data Translation: Now works wtih DT3120 frame grabber. Frame rate when exposures are external triggered limited to 5 FPS.

WinFluor V3.9.6 7/1/18

Bug Fixes 

Photometrics Prime: Now triggers correctly without additional readout time and no longer misses trigger for second framke in sequence (CLEAR_MEVER now used instead of CLEAR_PRESEQUENCE).

NI Interface cards: D/A waveforms now updated as double precision voltages to avoud scaling errors with USB 6002/3 devices.

Record Images & SIgnals: Floating point errors on some systems due to uninitialised floating point plotting buffers fixed,

WinFluor V3.9.5 27/11/17

Bug Fixes 

Export > Image Series: First file exported in PIC format no longer empty of images.

WinFluor V3.9.3 7/11/17

Bug Fixes 

Export>ROI Time Course: Divide by zero error which occurred when all ratio or Ca time courses were zero now prevented.

WinFluor V3.9.2 02/10/17

Bug Fixes 

Record Images & Signals: Live ratio trace now works correctly again.

Record Image: Optoscan monochromator now works again.  Scroll bar out of range errors blocked.

PVCAM cameras: Camera shutter (if present) now opened before start of capture sequence.

WinFluor V3.9.1 31/7/17

Bug Fixes 

File Export: Metamorph STK files now exported correctly again. BIGTIFF files> 4GB now exported correctly

WinFluor V3.9.0 14/7/17

Updates

Analogue Channels: Analogue PMT Fluorescence ratios can now be computed from Cairn Research Modular Photometry System.

Bug Fixes

LED Light Source: LEDs controlled by TTL digital lines now set correctly when inverted control selected (On=0V, Off=5V).

WinFluor V3.8.7 8/12/16

Bug Fixes

View Images: Multi-wavelength ROI time courses now plotted correctly again.

Cameras: Point Grey Grasshopper 3. Left and Top edges of CCD sub-region now selected correctly.

WinFluor V3.8.4 31/3/16 

Bug Fix

National Instruments Interface: PCIe-632X boards now recognised as having digital outputs capable of supporting digital stimuli or use as LED/camera control lines.

WinFluor V3.8.3 11/3/16 

Bug Fix

National Instruments Interface: Single Ended (RSE) analogue input mode now correctly selected (instead of Single Ended (NRSE) which was selected by mistake).

WinFluor V3.8.2 4/3/16 

Bug Fixes

Multiclamp 700A: Should now recognise Multiclamp 700A and read channel scaling factors again.

Andor Cameras: Access violation when loading settings from file fixed.

WinFluor V3.8.0 25/11/15 

Bug Fix

Record Images & Signals: Floating point divide by zero when no interface units available fixed.

WinFluor V3.7.9 24/11/15 

Updates

Interface Units: Limited support for digital outputs on USB-6002/3 devices now available. Single LEDs can be selected/turned on/off in Record Image and Record Images & Signals windows but time lapse and multi-wavelength imaging not supported.

WinFluor V3.7.8 19/11/15

Bug Fixes

Record Images & Signals: Floating point divide error in Record Images & Signals on some computers fixed.

Updates

Cameras: Point Grey Grasshopper 3 camera support added (using National Instruments NIMAQdx camera library).

Updates

Interface Units: Support for National Instruments USB-6001-3 interface units added. Analog channel and amplifier setup now on Analog Channel & Amplifiers page of Camera / System setup.

Thorlabs cameras: Unsupported is_devicefeatures() error messages now blocked with DCU cameras which do not support shutter modes.

Bug Fixes

LED light source: Inverted TTL LED control (0=ON,5V=OFF) now works correctly.

Detect Events: Event detection revised. Numerous minor bugs fixed.

Record Images & Signals: auto contrast feature now works correctly.

Updates

XY Stage: Now supports Thorlabs MLS203 joystick and saves positions set by joystick.

Updates

QImaging Cameras: Disable Exposure Time Checking option added to Camera Setup allowing checking of exposure interval against calculated readout time to be disabled.

Updates

Updates

Updates

UpdatesÂ

QImagjng Cameras: Absolute m inimum exposure time reduced to 10ms.

Updates

LED light source now supports up to 8 LEDs when using TTL digital output lines. LED intensity can now be set using Set Light Intensity dialog box in Record/Images & Record Images & Signal windows when using analog outputs to control LEDs.

Updates

QImaging OptiMOS: Exposure time operating in externally triggered exposure mode can now be reduced to 15 ms.

National Instruments IMAQ interfaces: Support added for ORCA-100 (not yet tested)

Bug Fixes:

File/Export Images: Files with long file paths no longer generate a "file not found" error preventing export to TIF and other file formats (since V3.5.9).

Bug Fixes

64 bit version now works with Andor SDK2 cameras.

64 bit version no longer crashes when imaging with Cairn Optoscan selected as light source.

Updates

Support for Thorlabs DCx series cameras added.

Bug Fixes

Exposure time now adjusted for Emission filter change time when emission filters in use (and no longer needs to be set to zero when filters not in use.).

WinFluor V3.6.4 9/7/14

Bug Fixes

MultiClamp 700: 64 bit version now works correctly.

WinFluor V3.6.3 9/7/14

Bug Fixes

MultiClamp 700: Updated to support V2.X versions of MultiClamp Commander.

Updates

PVCAM & NIMAQdx: Individual cameras can now be selected when multiple cameras are available on interface.

WinFluor V3.6.2 9/7/14

Bug Fixes

Record windows: Calibration bars now scaled by lens magnification.

PVCAM: Error message appearing when some cameras restarted now blocked.

QImaging: 64 bit library now detected correctly.

Load/Save Settings: Settings now saved in C:\Users\Public\Documents\WinFluor rather than program folder.

WinFluor V3.6.1 23/6/14

Updates

PVCAM: Support for QImaging OptiMOS added. Improvements to CoolSnap support.

Disk file writing made more efficient. Higher frame rates can now be recorded.

Bug Fixes

Shutter blinking at start of bursts in Time Lapse+Burst recording mode fixed by opening shutter 10 seconds before burst.

Errors in recording duration and frame interval when Split Image mode selected fixed.

Real time course plots can now have more than 100000 frames.

WinFluor V3.6.0 16/5/14

WinFluor V3.5.9 15/4/14

WinFluor V3.5.8 3/4/14

Updates

Light Source: Bulb exposure mode added and % exposure option added to excitation light wavelength control allowing camera exposure to be varied with excitation light wavelength. Emission filter control added.

Split Image: Split image mode added, allowing upper and lower halves of image to be treated as separate image frames.

PVCAM: Now supports 64 bit DLL library.

Bug Fixes

Hamamatsu DCAM: 64 bit support updated. NOT TESTED YET.

WinFluor V3.5.7 20/2/14

Updates

64 bit version now available.

Bug Fixes

Support for Andor Zyla and Hamamatsu improved, number of bugs and limitations fixed. Larger image buffer size allocated.

Bug introduced in V3.5.4 fixed. AVI file creation (Create Movie) now works again. Access violations in Time Course Analysis fixed.

WinFluor V3.5.4 9/11/13

Bug Fix

Hamamatsu C4480: Failure to detect .CNF files (since V3.50) now fixed. Should now work correctly with Hamamatsu C4880 camera.

WinFluor V3.5.2 7/11/13

Light source shutter: Shutter opening time can now be defined in setup allowing shutter to be opened at preset time before exposure in time lapse mode.

LED Light Source: LED on/off voltages can now be set when using digital outputs (On=5V, Off=0V for active high LEDS On=0V, Off=5V for active low LEDS).

Z stage: Z stage movements now take place AFTER image (or series of images in multi-wavelength mode) are acquired in time lapse recording mode. In continuous recording mode, Z stage is moved at preset time before end of image acquisition frame. Illumination can be turned off or exposure terminated during Z stage movement period. Z step time defined in Z stage setup.

Andor Ixon Cameras: Camera readout noise can now be optimised by adjusting Readout A/D gain and vertical shift timing in Setup/Extra Camera Settings.

WinFluor is now compiled using Embarcadero Delphi XE3 development system

WinFluor V3.4.9 9/7/13

WinFluor V3.4.8 4/6/13

WinFluor V3.4.7 3/6/13

Updates:

Andor Cameras:Support for Andor Zyla sCMOS camera using SDK3 library added (may also support Neo but not tested).

Light Sources:Support for PTI/Till monochromators + additional LED/Laser light sources added.

WinFluor V3.4.6 24/04/13

Bug Fixes

Biorad/PraireView recording: Bug fix in NIDAQMX_MemoryToDAC() to avoid access violations when A/D restarted. Real time display sweep now correctly synchronised to incoming signal

Stimulus Protocols:Default protocol folder now selected in folder in INI file not found. SelectDirectory() dialog now used to set voltage protocol folder.

WinFluor V3.4.5 03/04/13

Updates:

Record Image & Signals:Image capture now functions when camera triggered by digital line and no analog outputs are in use. Small rectangles at corners of image selection region added to make it more visible. Camera now restarted when excitation turned on/off to speed up change. Camera restarted when new file created.

QImaging Cameras:Trigger input connection now included in help file.

WinFluor V3.4.4 30/01/13

Updates:

Support for voltage-driven piezo Z stage controllers added. Z stacks can now be acquired in Images & Signals recording module.

Camera/System Setup window now split into tabbed pages.

WinFluor V3.3.8 30/7/12

Bug Fixes:

QCAM:Error when opening QImaging Rolera XR fixed

Updates:

Voltage and Digital stimuli can now be started and stopped without restarting camera. Allows linked protocols during recording without interrupting recording of images.

WinFluor V3.3.7 30/4/12

Bug Fixes:

NI Interface units:Digital stimulus output lines of X series (USB-6341 etc) interface boards now detected.

Andor cameras:Folder containing detector.ini file required for older Ixon cameras now located correctly.

WinFluor V3.3.0 27/7/11

Updates:

Support for Data Translation frame grabber cards added.

Export ROI Time Course: All ROIs can be exported simultaneously tooneor moreexport files.

Bug Fix:

Size of files larger than 2GB now determined correctly, preventing "No.of frames does not match size of file." warning message when these files are reloaded.

WinFluor V3.2.25 15/12/10

Updates:

National Instruments NIMAQ-DX library supported, allowing (DirectShow, GIGE, USB and firewire cameras)

Bug Fixes:

PVCAM cameras: Cameras now correctly operate in frame transfer mode, allowing full frame speed of camera to be achieved.

WinFluor V3.2.24 1/11/10

Updates:

Andor Ixon cameras: Conventional CCD mode now supported in addition to EMCCD. A/D converter channels can now also be selected.

Bug Fixes:

ROI time course now updated automatically when ROI's added/changed.

WinFluor V3.2.19 27/01/10

Updates:

No. of multi-wavelength sequences increased to 10 andcan be selected from drop-down menu in recording window.

Automatic contrast adjust added to live, recording and playback windows.

Fluorescence time course of a selected ROI can now be exported to data file.

Export to IGOR IBW data files now supported.

Bug Fixes:

Ion concentration images produced using binding equation now work correctly.

WinFluor V3.2.16 10/9/09

Updates:

Regions of Interest:User-defined, irregular shaped, lines and region ROIs added. ROIs now added to display by druag & drop and can be moved around after placement on image.

Line Profile module:Edge tracking feature added to line profile module, allowing location ofwavefronts or movement of cell edges to be tracked. Line profiles can be computed from new irregular line ROI.

Average / Subtract Frames: Images of the inter-frame pixel intensity differences can now be produced.

Hamamatsu Image-EM:EMCDD mode can now be disabled (this feature not tested).

WinFluor V3.2.15 22/5/09

Updates:

Cooling and fan of Andor Ixon cameras can be turned off using Additional Camera Settings dialog box.

A shutterblanking period (definedby "Frame blanking period" in the Light Source group in Camera/System Setup) can be placed at the end of each camera exposure frame to turn off excitation light during camera readout.

PCO Sensicams can now be set to 8X8 binning.

Bug Fix:

Ratio images created using "Create Ratio/Ion Images" can now be exported as TIFF files.

WinFluor V3.2.14 17/4/09

Bug Fix

Image repetition glitch in Live Image window fixed.

External triggered image capture now works with Hamamatsu Image-EM.

Selection of CCD sub-areas now more precise and does not shrink with repeated selection of same area.

WinFluor V3.2.13 18/3/09

Bug Fix

Optoscan + Lasers light source: Laser #1 shutter should now work. Monochromator slit widths should remain unchanged when laser selected.

WinFluor V3.2.9 11/3/09

Bug Fix

Update with possibles fixes for bugs in Optoscan + Lasers light source.

WinFluor V3.2.9 12/3/09

Update

Optoscan + Lasers light source: DAC lines for controlling lasers can now be definedas a separate range from DAC lines controlling monochromator. Allows monochromator and lasers to ve controlled from separate NI boards.

Bug Fix

Unwanted voltage pulse to 0mV no longer occurs when switching between seal test, live and recording windows, and starting/stopping stimulus pulses (occurred when voltage stimulus channel 1 was undefined).

WinFluor V3.2.8 17/0 2/09

Bug Fix

National Instruments PCI/USB-625X boards now recognised to have digital waveform capabilities.

WinFluor V3.2.6 26/01/09

Updates

Shutter closed wavelengthfor monochromators can now be set by user (Camera/System setup page).

Extra readout time (subtracted from exposure) can beallowed for externally triggered cameras (Camera/System setup page).

Shading correction function enhanced and added to recorded images. Background subtraction image created and can be removed from images in Display Stored Images

Bug fixes

Andor DLL library atmcd32d.dll longer needs to be copied into c:\Winfluor folder.

WinFluor V3.2.5 27/08/08

Updates

Shading correction function (subtracts smoothed variations in background) added to Live Images window.

WinFluor V3.2.4 06/08/08

Updates

Support for two patch clamps added.

Photo stimulus module for Prairie Ultima 2-galvo system added (needs additional testing).

Signal time course plots now have better chosen oY axis calibration ticks. Channels can be individually removed from display and resized.

Bug Fixes

Displayof line scan images now works correctly.

WinFluor V3.1.8 20/5/08

Updates

Live image window added allowing camera image to be observed simultaneously with patch clamp seal test window.

Support for Sutter DG-4 filter changed added.

Support for Hamamatsu C4880 camera with DCAM library added.

No of wavelengths in excitation wavelength table increased to 20. Multiple rates now possible within multi-wavelength excitation sequences. Selected wavelengths can be applied at fraction (up to 1/100) of other wavelengths.

WinFluor V3.0.9 29/8/07

Bug Fixes

Digital stimulus waveforms no longer produce memory errors when long duration waveforms generated.

No. of increments in multi-step stimulus protocols no longer reset to one when single step or ramp waveforms edited.

Seal test pulses can now generated correctly without closing Live Imaging window.

WinFluor V3.0.8 21/8/07

Updates

Digital pulse waveform with incrementing delay added waveform editor.

WinFluor V3.06 31/7/07

Bug Fixes

Export to Metamorph STK format files now works correctly

WinFluor V3.05 31/7/07

Updates

Fluorescence ratio time course plots now available both in live and stored file windows, line scans and detected events.

Vertical magnification of time course improved. Can now be set using buttons at right side of display or by directly highlighting a region with the mouse.

Event detector options improved. Events can be detected using relative as well as absolute levels.

User guide now supplied with program.

Bug Fixes

Time lapse image capture now works reliably at capture rates up to 3X exposure time.

Many minor bugs fixed.